Enzyme

Enzyme Action Objectives: 1.) Identify the class of macromolecules to which peroxidase belongs and the subunits that comprise it. 2.) Name substrate and products of the peroxidase=catalyzed reaction. 3.) beg off the role of guaiacol in this experiment 4.) condition enzyme; activation, zero, active site, ph, and denaturation. 5.) Distinguish betwixt oxidation/reduction, activation energy/catalysis, substrate/product, and hydrogen bleach/peroxidase. 6.) Define the term optimum with respect to peroxidase activity 7.) Explain wherefore peroxidase is a essential enzyme for every aerobic or atomic number 8 utilizing cells. Procedure: We utilize 3 pull outning play electron pipages per plump. resistance 1 was filled with 0.1 ml of guaiacol, 1.0 ml of peroxidase (Turnip extract), and 8.9 ml of distilled water. supply 2 contained; 0.1 ml f guaiacol, 0.2 ml of enthalpy peroxide (H2 O2), and 4.7ml of distilled water. pipework 3 was filled with 1.0ml of peroxidas e and 4.0ml of distilled water. These tierce tubes acted as our baseline. Tube 1 was employ to fill up a smaller test tube which was placed into a spectrophotometer, pastce zeroed it out. The tube was then removed and cleaned out.

Tubes 2 and 3 were entangled unneurotic (at this point a stop watch is started) then were used to fill another small test tube. This test tube was placed in the spectrophotometer, every thirty seconds a variation was taken, this continued on for three transactions. After three minutes the tube was removed and cleaned. This process was iterate for runs 2, 3, and 4. for run 5a. through 5d. each tube was placed in a varied temperatur! e changing environment (freezing, cooling, heating and boiling). These runs were only preserve at their start times and at three minutes. These processes were all repeated until round 8. Each round varied in enzyme used but had same process. Data/Observations : questions and data on attachedIf you necessity to get a full essay, order it on our website: OrderCustomPaper.com

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